Research Article
Performance Evaluation of the HemoTypeSCTM Rapid Screening Test for the Detection of Hemoglobin A, S, and C Variants at the Fann National University Hospital
Sene Moustapha Ndep*
,
Kandji Pape Matar,
Barry Nene Oumou Kesso,
Thioune Ndeye Mareme,
Ndiaye Siny,
Djite Moustapha,
Ndour El Malick,
Gueye-Tall Fatou,
Gueye Papa Madieye
Issue:
Volume 14, Issue 4, December 2026
Pages:
89-93
Received:
7 September 2026
Accepted:
20 September 2026
Published:
23 October 2026
DOI:
10.11648/j.ab.20261404.11
Downloads:
Views:
Abstract: Hemoglobinopathies, particularly sickle cell disease, pose major public health challenges in resource-limited settings, where delayed diagnosis contributes to high morbidity and mortality. As the world's most common monogenic disorder, the vast majority of affected individuals reside in sub-Saharan Africa. This study evaluated the performance of the HemoTypeSCTM rapid test for detecting hemoglobin A, S, and C variants, in accordance with the NF EN ISO 15189 standard. A prospective analytical study was conducted at the Biochemistry-Hematology Laboratory of Fann National University Hospital Center. Patients referred for hemoglobin electrophoresis were tested using HemoTypeSCTM, with the Emmel test and capillary electrophoresis (Capillarys 3 octa, Sebia) as comparative methods. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and overall agreement were calculated with 95% confidence intervals. Among the 126 samples, 19 had normal profiles and 107 had hemoglobinopathies. Compared with the Emmel test, HemoTypeSCTM showed 100% sensitivity and specificity. Compared with capillary electrophoresis, sensitivity was 98.16%, specificity 100%, PPV 100%, and NPV 90.48%. Two discrepancies in fine typing were observed among the 107 abnormal profiles, with no missed abnormal profiles. HemoTypeSCTM is a rapid, reliable, and easy-to-use test for first-line sickle cell disease screening in resource-limited settings. Positive results should be systematically confirmed by electrophoresis.
Abstract: Hemoglobinopathies, particularly sickle cell disease, pose major public health challenges in resource-limited settings, where delayed diagnosis contributes to high morbidity and mortality. As the world's most common monogenic disorder, the vast majority of affected individuals reside in sub-Saharan Africa. This study evaluated the performance of th...
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